Transformation of chenodeoxycholic acid to ursodeoxycholic acid in patients with Crohn's disease.

Abstract

In vivo 7 beta-epimerization of chenodeoxycholic acid to ursodeoxycholic acid and the role of 7-ketolithocholic acid as an intermediate in this biotransformation were studied in 11 patients with Crohn's disease and in 5 healthy volunteers. The incorporation of deuterium into biliary ursodeoxycholic acid and 7-ketolithocholic acid was determined by computed gas chromatography-mass fragmentography after ingestion of a dideuterated chenodeoxycholic acid, chenodeoxycholic-11,12-d2 acid. The incorporation of deuterium into ursodeoxycholic acid increased to a peak level at 48 h in the patients with Crohn's disease, but was delayed in healthy volunteers. In 8 patients and 2 healthy controls there were small amounts of 7-ketolithocholic acid in bile. The incorporation of deuterium into 7-ketolithocholic acid was confirmed in only 2 patients and the peak level was noted at 48 h. These observations suggest that 7-ketolithocholic acid is an intermediate of this biotransformation in patients with Crohn's disease.

• Authors: H Miwa, M Yamamoto, T Nishida, T Yao
• Journal: Gastroenterology (Gastroenterology) Vol. 90 Issue 3 Pg. 718-23 (Mar 1986) ISSN: 0016-5085 [Print] United States
• PMID: 3943699 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bile Acids and Salts
• Deoxycholic Acid
• Chenodeoxycholic Acid
• 7-ketolithocholic acid
• Lithocholic Acid
• Ursodeoxycholic Acid
• Deuterium

Topics

• Adolescent
• Adult
• Bile (analysis)
• Bile Acids and Salts (analysis)
• Chenodeoxycholic Acid (metabolism)
• Crohn Disease (metabolism)
• Deoxycholic Acid (analogs & derivatives)
• Deuterium
• Female
• Gas Chromatography-Mass Spectrometry
• Humans
• Lithocholic Acid (analogs & derivatives, metabolism)
• Male
• Middle Aged
• Ursodeoxycholic Acid (metabolism)