Expression of Ia antigens in a murine T-lymphoma variant.

Abstract

ESb, a cellular high metastatic variant derived from the murine T-cell lymphoma L5178Y (Eb), was found to synthesize Ia antigens. Ia-specific antibodies reacted with the ESb cells and precipitated Ia-like molecules from them. Two-dimensional gel electrophoretic analysis of immunoprecipitates of metabolically labeled ESb cells indicated that the Ia molecules on ESb were indistinguishable from those on murine B-cells. No Ia antigens were detectable on the parental tumor line Eb. Treatment with recombinant interferon-gamma (IFN-gamma) caused enhancement of class I histocompatibility antigen expression on Eb and ESb tumor lines. In ESb cells the expression of Ia and of Ia-associated invariant chain (Ii) was also increased upon IFN-gamma treatment. No induction of either Ia and Ii antigens was observed upon IFN-gamma treatment of the Eb line. These studies demonstrate a substantial difference between the Eb and ESb tumor lines with respect to: (i) constitutive expression of class II major histocompatibility antigens, and (ii) response to IFN-gamma treatment.

• Authors: L Graf, N Koch, V Schirrmacher
• Journal: Molecular immunology (Mol Immunol) Vol. 22 Issue 12 Pg. 1371-7 (Dec 1985) ISSN: 0161-5890 [Print] England
• PMID: 3938525 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Histocompatibility Antigens Class II
• Interferon-gamma

Topics

• Animals
• Antigens, Neoplasm (analysis)
• Cell Line
• Electrophoresis, Polyacrylamide Gel
• Histocompatibility Antigens Class II (analysis)
• Interferon-gamma (pharmacology)
• Lymphoma (immunology)
• Major Histocompatibility Complex
• Mice
• Mice, Inbred DBA
• Radioimmunoassay
• T-Lymphocytes