The molecular weight-dependent distribution of urinary glycosaminoglycans in Werner's syndrome.

Abstract

The macromolecular AGAG in the urine of patients with Werner's syndrome were analyzed by enzymatic methods after digestion with chondroitinases and Streptomyces hyaluronidase. The molecular weight-dependent distribution of the urinary AGAG has been determined by gel filtration on a Sephadex G-100 column. The distribution of HA and HS was predominant in the macromolecular fractions. Chondroitin sulfate isomers were prominent in the low molecular weight fractions but the ratio of the 4-type to the 6-type increased with decreasing molecular weight. These observations indicated that Werner's syndrome is a metabolic disorder of the molecular weight-dependent AGAG composition.

• Authors: K Murata
• Journal: Biochemical medicine (Biochem Med) Vol. 34 Issue 3 Pg. 251-8 (Dec 1985) ISSN: 0006-2944 [Print] United States
• PMID: 3938279 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycosaminoglycans
• Dermatan Sulfate
• Hyaluronic Acid
• Heparin
• Chondroitin Sulfates

Topics

• Adult
• Chondroitin Sulfates (analysis)
• Chromatography, Gel
• Dermatan Sulfate (analysis)
• Electrophoresis, Cellulose Acetate
• Female
• Glycosaminoglycans (urine)
• Heparin (analysis)
• Humans
• Hyaluronic Acid (analysis)
• Male
• Middle Aged
• Molecular Weight
• Werner Syndrome (urine)