Removal of non-A, non-B hepatitis virus from a concentrate of the coagulation factors II, VII, IX and X by hydrophobic interaction chromatography.

Abstract

The efficacy of a chromatographic procedure based on hydrophobic interaction chromatography to remove non-A, non-B (NANB) infection from a concentrate of coagulation factors II, VII, IX and X (Preconativ, KabiVitrum AB) was evaluated in chimpanzees. For this purpose, NANB infective human plasma (H-strain) was deliberately added to a solution of Preconativ (45 IU factor IX:C/ml) to reach a titre of NANB virus of greater than or equal to 10(2) chimpanzee infectious doses (CID)/ml. The NANB-contaminated preparation was chromatographed on octanohydrazide-Sepharose 4B at a high salt concentration. Groups of 2 chimpanzees were each inoculated intravenously with 45 IU factor IX:C of Preconative before and after this virus adsorption chromatographic step. The experimental animals remained free of any serological and biochemical evidence of hepatitis during a 12-month follow-up period. One of the two control animals developed clear evidence of NANB hepatitis.

• Authors: M Einarsson, A M Prince, B Brotman
• Journal: Scandinavian journal of infectious diseases (Scand J Infect Dis) Vol. 17 Issue 2 Pg. 141-6 ( 1985) ISSN: 0036-5548 [Print] England
• PMID: 3927477 (Publication Type: Journal Article)

Chemical References

• Blood Coagulation Factors

Topics

• Animals
• Blood (microbiology)
• Blood Coagulation Factors (isolation & purification)
• Chromatography, Agarose
• Hepatitis C (prevention & control)
• Hepatitis Viruses (isolation & purification)
• Humans
• Pan troglodytes
• Transfusion Reaction