[Immunoluminescent detection of antibodies to testicular antigens in experimental autoimmune orchitis].

Abstract

Antibodies interacting with spermatid structures in the testis sections and with acrosome and caudal regions of spermatozoa in the smears of sperm were determined in the sera of guinea pigs and mice with experimental autoimmune orchitis by the indirect immunofluorescent method. The antibodies were found in mice from the 14th to the 30th day and in guinea pigs from the 14th to the 60th day after immunization. Circulation time and the level of the fluorescent antibodies were significantly decreased in immunized female guinea pigs and mice. Fluorescent antibodies did not show cytotoxic effect and belonged to the IgG fraction. The fluorescent antibodies of guinea pigs did not react with the mouse testicular antigens in cross reaction. The analogous results were seen in the cross reaction of the mouse antibodies with the guinea pig antigens.

• Authors: R P Ogurtsov, Iu N Zubzhitskii
• Journal: Biulleten' eksperimental'noi biologii i meditsiny (Biull Eksp Biol Med) Vol. 88 Issue 12 Pg. 695-8 (Dec 1979) ISSN: 0365-9615 [Print] Russia (Federation)
• Vernacular Title: Immunoliuminestsentonoe vyiavlenie antitel k antigenam semennika pri éksperimental'nom autoimmunnom orkhite.
• PMID: 391295 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antibodies
• Antigens

Topics

• Animals
• Antibodies (analysis)
• Antigens (immunology)
• Autoimmune Diseases (immunology)
• Cytotoxicity Tests, Immunologic
• Female
• Fluorescent Antibody Technique
• Guinea Pigs
• Immunization
• Male
• Mice
• Mice, Inbred C57BL
• Orchitis (immunology)
• Testis (immunology)
• Time Factors