Induction of SOS responses in Escherichia coli by Panfuran-S, 3-di(hydroxymethyl)amino-6-(5-nitro-2-furylethenyl)-1,2,4-triazine.

Abstract

The inducibility of SOS responses by Panfuran-S, which has been used as an antimicrobial medicine in Japan, was studied in Escherichia coli cells having different DNA-repair capacities for UV lesions. Panfuran-S induced mutations at high frequencies in uvrA and the wild-type strains, and significant killing effects of Panfuran-S were detected in DNA-repair-deficient strains, uvrA and recA. The effective prophage induction was detected in two kinds of lambda-lysogenized cells treated with Panfuran-S. The expression of the umuC+ gene was apparently induced in uvrA and the wild-type strains, but not induced in lexA and recA strains. In particular, high inducibility of the gene expression was detected in uvrA strain as compared with the wild-type strain. From these results, we conclude that Panfuran-S is a DNA-damaging agent and may induce the error-prone SOS responses.

• Authors: T Ohnishi, Y Harada, Y Ohashi, Y Konishi, K Nozu
• Journal: Mutation research (Mutat Res) Vol. 146 Issue 3 Pg. 243-7 (Nov 1985) ISSN: 0027-5107 [Print] Netherlands
• PMID: 3903488 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Triazines
• dihydroxymethylfuratrizine

Topics

• Anti-Bacterial Agents (pharmacology)
• DNA Repair (drug effects)
• Escherichia coli (drug effects, genetics)
• Genes, Bacterial
• Mutation
• Triazines (pharmacology)