Abstract |
We studied antibodies to rheumatoid arthritis nuclear antigen (RANA) by the Ouchterlony method in 0.5% agarose plates, using soluble antigen extracted with 0.25 M sucrose solution from cultured Raji cells. Anti-RANA antibody was found in sera from 24 to 40 (60%) patients with rheumatoid arthritis (RA), from 4 of 20 (20%) patients with systemic lupus erythematosus (SLE), and from 2 of 30 (7%) healthy controls. When sucrose extracts from BJAB, Ramos, and JM cells were used as the cellular antigens, no anti-RANA precipitin lines were detected. Indirect immunofluorescence study, using Raji cells or human B lymphocytes transformed by EB virus as substrate tissues, demonstrated anti-RANA antibody as fine speckled nuclear staining. Although RA patients with positive anti-RANA antibody usually had high titers of anti-Epstein-Barr nuclear antigen (EBNA) and anti-viral capsid antigen (VCA) IgG antibodies, the Wilcoxon ranks sum test showed no close statistical correlation between the presence of anti-RANA antibodies and the titers of anti-EBNA or anti-VCA ( IgG) antibodies. These data showed that the incidence of positivity of anti-RANA antibodies in Japanese RA patients is almost the same as that in American and European RA patients.
|
Authors | K Nakabayashi, M Saito, T Nagasawa, M Takada |
Journal | Rheumatology international
(Rheumatol Int)
Vol. 5
Issue 2
Pg. 61-7
( 1985)
ISSN: 0172-8172 [Print] Germany |
PMID | 3885372
(Publication Type: Journal Article)
|
Chemical References |
- Antibodies
- Antibodies, Anti-Idiotypic
- Antigens
- Antigens, Viral
- Autoantigens
- Epstein-Barr Virus Nuclear Antigens
- Immunoglobulin G
- rheumatoid arthritis nuclear antigen
|
Topics |
- Adult
- Antibodies
(analysis)
- Antibodies, Anti-Idiotypic
(analysis)
- Antigens
(immunology)
- Antigens, Viral
(immunology)
- Arthritis, Rheumatoid
(immunology, physiopathology)
- Autoantigens
(immunology)
- Capsid
(immunology)
- Epstein-Barr Virus Nuclear Antigens
- Fluorescent Antibody Technique
- Humans
- Immunoglobulin G
(immunology)
- Lupus Erythematosus, Systemic
(immunology, physiopathology)
- Middle Aged
|