Interferon-induced protein Mx accumulates in nuclei of mouse cells expressing resistance to influenza viruses.

Abstract

In mouse cells carrying the dominant influenza resistance allele Mx+ (but not in Mx- cells) interferon-alpha/beta (IFN) induces an efficient antiviral state against influenza viruses and, concomitantly, the synthesis of a 75,000-Da protein (protein Mx). Here, indirect immunofluorescence using monoclonal antibodies was used to demonstrate that protein Mx accumulates in the nucleus of IFN-treated Mx+ cells, suggesting a nuclear site of action. Protein Mx is present in the nucleus of untreated influenza virus-resistant macrophages freshly explanted from the peritoneal cavity of Mx+ mice but is lost with time in culture when peritoneal macrophages become permissive for influenza virus.

• Authors: P Dreiding, P Staeheli, O Haller
• Journal: Virology (Virology) Vol. 140 Issue 1 Pg. 192-6 (Jan 15 1985) ISSN: 0042-6822 [Print] United States
• PMID: 3880978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Interferon Type I
• Mx1 protein, mouse
• Myxovirus Resistance Proteins
• Proteins
• GTP-Binding Proteins

Topics

• Alleles
• Animals
• Antibodies, Monoclonal
• Cell Nucleus (metabolism)
• Fluorescent Antibody Technique
• GTP-Binding Proteins
• Immunity, Innate
• Interferon Type I (immunology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Mice, Inbred Strains
• Myxovirus Resistance Proteins
• Orthomyxoviridae (immunology)
• Proteins (genetics)
• Species Specificity