The
tremors induced by
harmine, LON-954 (N-carbamoyl-2-(2,6-dichlorophenyl)acetamidine hydrochloride) and
5-hydroxytryptophan (5-HTP) were studied in control rats and in rats withdrawn for 16-48 hrs from 6 to 9 days'
ethanol administration. The frequencies and the intensities of the
tremors were determined electronically. In control rats the frequency spectra of the
tremors induced by
harmine (20 mg/kg) and LON-954 (10 mg/kg) showed a narrow peak frequency at about 10 Hz.
Atropine (1.2 mg/kg) altered neither the frequency nor the intensity of these
tremors.
5-HTP (50 mg/kg) when given 3.5 hrs after
iproniazid (100 mg/kg) induced
tremor with peak frequencies at 6-7 Hz and 12 Hz. In
ethanol-withdrawn rats treated with
harmine or LON-954 the frequency analysis of
tremor revealed a narrow peak frequency at about 12 Hz, which was neither the characteristic frequency of
ethanol withdrawal
tremor (6 Hz) nor that of
harmine or LON-954 (10 Hz). The intensity of both
harmine- and LON-954-induced
tremor was significantly increased in
ethanol-withdrawn rats. The
ethanol-withdrawn rats were markedly sensitized to the effect of iproniazid+
5-HTP, shown by deaths. The peak frequencies of this
tremor were the same as those in control rats. The results suggest that
harmine-induced
tremor involves a dopaminergic-5-HT'ergic imbalance and the
tremor induced by LON-954 a dopaminergic-
cholinergic imbalance in the brain. The
tremor in
ethanol-withdrawn rats seems to be mediated by alterations in the activity of the cerebral 5-HT'ergic system.