1-Aminooxy-3-aminopropane, a new and potent inhibitor of polyamine biosynthesis that inhibits ornithine decarboxylase, adenosylmethionine decarboxylase and spermidine synthase.
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| Abstract |
1-Aminooxy-3-aminopropane was shown to be a potent competitive inhibitor (Ki = 3.2 nM) of homogenous mouse kidney ornithine decarboxylase, a potent irreversible inhibitor (Ki = 50 microM) of homogeneous liver adenosylmethionine decarboxylase and a potent competitive (Ki = 2.3 microM) of homogeneous bovine brain spermidine synthase. It did not inhibit homogeneous bovine brain spermine synthase and it did not serve as a substrate for spermidine synthase. The compound did not inhibit tyrosine aminotransferase, alanine aminotransferase or aspartate aminotransferase, which are pyridoxal phosphate-containing enzymes like ornithine decarboxylase. The inactivation of adenosylmethionine decarboxylase was partially prevented by pyruvate, which is the coenzyme of adenosylmethionine decarboxylase, and by the substrate, adenosylmethionine. 1-Aminooxy-3-aminopropane at 0.5 mM concentration inhibited the growth of HL-60 promyelocytic leukemia cells and this inhibition was prevented by spermidine but not by putrescine.
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| Authors | R M Khomutov, T Hyvönen, E Karvonen, L Kauppinen, T Paalanen, L Paulin, T Eloranta, R L Pajula, L C Andersson, H Pösö |
| Journal | Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 130 Issue 2 Pg. 596-602 (Jul 31 1985) ISSN: 0006-291X [Print] United States |
| PMID | 3861182 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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