Abstract |
The tRNA methyltransferase activities of C57BL/6J, C57L/J, C58/J, AKR/J, and C3H/HeJ inbred mice were studied with the use of various amino acid-specific Escherichia coli tRNA's as substrates. Mice from two strains with high incidence of spontaneous leukemia (AKR/J and C58/J) exhibited levels of liver N2-guanine tRNA methyltransferase II (N2-MeGII) activity that were double those of two strains of mice with low incidence of spontaneous leukemia (C57BL/6J and C57L/J). Activities of liver and kidney N2-MeGII of the high spontaneous hepatoma strain C3H/HeJ were also found to be twice as high as those of C57BL/6J mice. The activities of other tRNA base-specific liver tRNA methyltransferases were very similar in all strains studied. The N2-MeGII activity of the F1 progeny of a cross between C57BL/6J and C3H/HeJ showed levels of activity intermediate to those of the parental strains. Activities of liver N2-MeGII of two inbred strains of mice that differ in their H-2 haplotype (C57BL/10SnJ and the congenic strain B10.BR/SgSnJ) were also compared. Both C57BL/10SnJ and B10.BR/SgSnJ strains exhibited low levels of liver N2-MeGII activity, indicating that H-2 does not directly control the activity of this enzyme.
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Authors | M Dizik, E Wainfan |
Journal | Journal of the National Cancer Institute
(J Natl Cancer Inst)
Vol. 74
Issue 1
Pg. 223-8
(Jan 1985)
ISSN: 0027-8874 [Print] United States |
PMID | 3855480
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- H-2 Antigens
- tRNA Methyltransferases
- tRNA (guanine(10)-N(2))-dimethyltransferase
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Topics |
- Animals
- Chromatography, High Pressure Liquid
- Crosses, Genetic
- H-2 Antigens
- Kidney
(enzymology)
- Liver
(enzymology)
- Mice
- Mice, Inbred Strains
- Species Specificity
- tRNA Methyltransferases
(metabolism)
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