[Cell-killing effect in vitro of prostaglandin derivatives on an established human bladder cancer cell line, KK-47].

Abstract

The cytotoxic effects of the prostaglandin derivatives, NP01A, NP07A and NP13A (Nihoniyakuhin Kogyo Co., Ltd., Toyama, Japan) on asynchronous cells of an established human bladder cancer cells line, KK-47, have been investigated using an in vitro colony formation technique. The following results obtained: NP01A-and NP13A-treated cells presented survival curves showing an exponential decrease with increasing drug concentrations. NP07A-treated cells demonstrated a survival curve bearing a prominent shoulder at considerably high drug concentrations. Using these survival curves, the 50% and 90% cell growth inhibition concentrations (IC50 and IC90) of the derivatives were computed. NP13A showed the highest cell-killing effect in terms of IC50 or IC90. According to a statistical analysis of variances followed by unpaired t-test, NP07A showed a higher cell-killing effect than that of NP01A (P less than 0.01). Based on a therapeutic index obtained from the ratio of clinical dosage to IC90, the derivatives proved to be of value in further in vivo studies.

• Authors: T Uchibayashi, H Hisazumi
• Journal: Gan to kagaku ryoho. Cancer & chemotherapy (Gan To Kagaku Ryoho) Vol. 12 Issue 8 Pg. 1669-74 (Aug 1985) ISSN: 0385-0684 [Print] Japan
• PMID: 3839645 (Publication Type: English Abstract, Journal Article)

Chemical References

• Prostaglandins E, Synthetic
• NP 13 A
• NP 01A
• NP 07 A
• Alprostadil

Topics

• Alprostadil (analogs & derivatives)
• Cell Division (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Cells, Cultured
• Drug Resistance
• Humans
• Prostaglandins E, Synthetic (pharmacology)
• Urinary Bladder Neoplasms (pathology)