Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups.

Abstract

The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complementation groups tested.

• Authors: A J de Jonge, W Vermeulen, W Keijzer, J H Hoeijmakers, D Bootsma
• Journal: Mutation research (Mutat Res) 1985 Jun-Jul Vol. 150 Issue 1-2 Pg. 99-105 ISSN: 0027-5107 [Print] Netherlands
• PMID: 3839045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Multienzyme Complexes
• UV endonuclease
• DNA
• Endodeoxyribonucleases
• deoxyribonuclease V
• N-Glycosyl Hydrolases

Topics

• Cells, Cultured
• DNA (biosynthesis, radiation effects)
• DNA Repair
• Endodeoxyribonucleases (administration & dosage)
• Genetic Complementation Test
• Humans
• Micrococcus (enzymology)
• Microinjections
• Multienzyme Complexes (administration & dosage)
• N-Glycosyl Hydrolases
• Ultraviolet Rays
• Xeroderma Pigmentosum (enzymology, genetics)