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Serotyping of dengue viruses by an enzyme-linked immunosorbent assay.

Abstract
We have developed a sandwich enzyme-linked immunosorbent assay for serotyping dengue viruses. In this assay, we used antibody from dengue hemorrhagic fever patients for detection of flavivirus common antigens to confirm virus isolation in C6/36 cells and that from hyperimmune mouse ascitic fluids for serotyping. The anti-dengue antibody was immobilized on microplate wells for capturing of dengue antigens, which were then sandwiched with the same biotinylated antibody. Then the biotin in the solid phase was detected with peroxidase-conjugated streptavidin. We found that all the dengue strains tested were unequivocally identified by this method.
AuthorsM Nawa, Y Ichikawa, S Inouye
JournalJapanese journal of medical science & biology (Jpn J Med Sci Biol) 1985 Oct-Dec Vol. 38 Issue 5-6 Pg. 217-21 ISSN: 0021-5112 [Print] Japan
PMID3834210 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Viral
  • Antigen-Antibody Complex
  • Antigens, Viral
  • Biotin
Topics
  • Antibodies, Viral
  • Antigen-Antibody Complex
  • Antigens, Viral (analysis)
  • Biotin
  • Dengue (immunology)
  • Dengue Virus (classification, immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Serotyping

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