Bryodin, a ribosome-inactivating protein from the roots of Bryonia dioica L. (white bryony).

Abstract

Bryodin is a strongly basic (pI greater than or equal to 9.5) glycoprotein (neutral sugar content 6.3%) with Mr 30,000, purified from the roots of Bryonia dioica (white bryony). This protein inhibits protein synthesis by a rabbit reticulocyte lysate with and ID50 (concentration causing 50% inhibition) of 0.12 nM (3.6 ng/ml) and has much less effect on protein synthesis by whole cells, with ID50 values ranging from 46 nM to 2.27 microM (1.4-67 micrograms/ml). Bryodin acts by inactivating ribosomes, with a less-than-equimolar ratio, which suggests a catalytic action. Bryodin decreases the number of local lesions induced by tobacco mosaic virus in the leaves of Nicotiana glutinosa. From all its properties, bryodin can be considered to be a ribosome-inactivating protein, similar to those already known [reviews: Barbieri & Stirpe (1982) Cancer Surveys 1, 489-520; Stirpe & Barbieri (1986) FEBS Lett. 195, 1-8].

• Authors: F Stirpe, L Barbieri, M G Battelli, A I Falasca, A Abbondanza, E Lorenzoni, W A Stevens
• Journal: The Biochemical journal (Biochem J) Vol. 240 Issue 3 Pg. 659-65 (Dec 15 1986) ISSN: 0264-6021 [Print] England
• PMID: 3827858 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Macromolecular Substances
• Plant Proteins
• Ribosome Inactivating Proteins, Type 1
• Toxins, Biological
• bryodin
• Poly U
• Phenylalanine
• DNA

Topics

• DNA (biosynthesis)
• Lymphocytes (drug effects, metabolism)
• Macromolecular Substances
• Phenylalanine (metabolism)
• Plant Proteins (isolation & purification, pharmacology)
• Plants (analysis)
• Poly U (pharmacology)
• Protein Biosynthesis
• Ribosome Inactivating Proteins, Type 1
• Ribosomes (drug effects, metabolism)
• Toxins, Biological