[Characteristics of intracellular metabolism of carbohydrates and amino acids in animals with thiamine deficiency].

Abstract

Vitamin B1 deficiency in mongrel albino rats induced by oxythiamine is concomitant with an increase in the ratio of free forms of NAD+/NADH, NADP+/NADPH in liver mitochondria. This is accompanied with a rise in steady-state concentrations of isocitrate, 2-oxoglutarate and with a decrease of malate in liver tissue, which testifies to the prominent regulatory role of the 2-oxoglutarate dehydrogenase complex in the intracellular metabolism under vitamin B1 deficiency. Moderate physical load causes a 10-fold increase in the steady-state concentration of pyruvate and lactate in the blood of thiamine-deficient animals, which seems to be due to the stimulation of glycolysis in the maintenance of energy homeostasis. The observed increase in the excretion of pyruvate, lactate, 2-oxoglutarate (30-fold against control) and pentose phosphates (3-fold) with urine, depending on the degree of vitamin B1 deficiency, points to one of essential mechanisms of cell metabolism stabilization under the given pathological condition.

• Authors: Z V Gorbach, S S Maglysh, L I Nefedov, Iu M Ostrovskiĭ
• Journal: Biokhimiia (Moscow, Russia) (Biokhimiia) Vol. 52 Issue 1 Pg. 42-52 (Jan 1987) ISSN: 0320-9725 [Print] Russia (Federation)
• Vernacular Title: Zakonomernosti vnutrikletochnogo metabolizma uglevodov i aminokislot u zhivotnykh pri nedostatochnosti tiamina.
• PMID: 3814653 (Publication Type: English Abstract, Journal Article)

Chemical References

• Amino Acids
• NAD
• Oxythiamine
• Acetone
• NADP

Topics

• Acetone (blood)
• Amino Acids (metabolism)
• Animals
• Carbohydrate Metabolism
• Citric Acid Cycle
• Glycolysis
• Liver (metabolism)
• Male
• NAD (metabolism)
• NADP (metabolism)
• Oxidation-Reduction
• Oxythiamine (pharmacology)
• Pentose Phosphate Pathway
• Physical Exertion
• Rats
• Thiamine Deficiency (metabolism)