Donor cornea contamination with McCarey-Kaufman medium preservation.

Abstract

A patient had corneal infection with Staphylococcus aureus after keratoplasty from tissue preserved in McCarey-Kaufman medium. The small, successfully treated corneal infection was from the same organism cultured from the tissue culture medium at the time of transplantation. Possible sources of donor-tissue contamination included the increase in ocular flora seen in postmortem eyes, blood-borne bacteria, lack of sterile technique in securing donor tissue, inadequate irrigation of the tissue, and inadequate use of topical antibiotics on the tissue. Preservation medium and specimens from medium-preserved and refrigerated corneas should be cultured before and during surgery.

• Authors: H Escapini Jr, R J Olson, H E Kaufman
• Journal: American journal of ophthalmology (Am J Ophthalmol) Vol. 88 Issue 1 Pg. 59-62 (Jul 1979) ISSN: 0002-9394 [Print] United States
• PMID: 380350 (Publication Type: Case Reports, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anti-Bacterial Agents
• Culture Media
• Gentamicins
• Bacitracin
• Methicillin

Topics

• Administration, Topical
• Anti-Bacterial Agents (pharmacology)
• Bacitracin (administration & dosage, therapeutic use)
• Cornea (microbiology)
• Corneal Transplantation
• Culture Media (standards)
• Drug Contamination
• Female
• Gentamicins (pharmacology)
• Humans
• Injections, Intravenous
• Keratitis (drug therapy, etiology)
• Methicillin (administration & dosage, therapeutic use)
• Microbial Sensitivity Tests
• Middle Aged
• Postoperative Complications (drug therapy, etiology)
• Staphylococcus aureus (drug effects, isolation & purification)
• Transplantation, Homologous