[Hybridomas synthesizing monoclonal antibodies to surface antigens of human lymphocytes].

Abstract

Three types of hybridomas were obtained by fusion of murine myeloma cells (NSI-1-Ag4-1) with splenocytes from mice immunized with human lymphoblastoid cells (RPMI-6410t line, acute myeloblastic leukemia). Hybridomas of the first type synthesize monoclonal antibodies Ma-1, which interact with 6410t-cells, but are not bound to the cells of human Burkitt lymphoma-Raji. Raji cells contain HLA-DRw5 and -DRw6 antigens on cell surface but there are no HLA-A2, -B7 and -B12 antigens (specific for 6410t). Thus, Ma-1 are probably derected against some of HLA antigens of loci A or B. Hybridomas of the second type synthesize Ma-2 antibodies which react with 6410t and Raji cells, but are not bound to peripheral blood lymphocytes (PBL). We suppose that Ma-2 antibodies to tumor specific antigens which have common antigen determinants both for Raji and RPMI-6410t cells. The third type of hybridomas synthesizes monoclonal antibodies Ma-3 reacting with all the three types of target cells: 6410t, Raji, and PBL. Ma-3 seems to be directed against human species-specific lymphocyte antigens which remained in 6410t and Raji cells.

• Authors: T M Seregina, M I Mekshenkov
• Journal: Tsitologiia (Tsitologiia) Vol. 28 Issue 9 Pg. 976-80 (Sep 1986) ISSN: 0041-3771 [Print] Russia (Federation)
• Vernacular Title: Gibridomy, sinteziruiushchie monoklonal'nye antitela k poverkhnostnym antigenam limfotsitov cheloveka.
• PMID: 3798564 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Surface

Topics

• Animals
• Antibodies, Monoclonal (analysis, biosynthesis)
• Antibody Specificity
• Antigens, Surface (analysis, immunology)
• Cell Line
• Humans
• Hybridomas (immunology)
• Lymphocytes (immunology)
• Mice