Detection of Cowdria ruminantium antigen and antibody during the course of heartwater disease in sheep by means of an enzyme-linked immunosorbent assay.

Abstract

An enzyme-linked immunosorbent assay was used to detect Cowdria ruminantium antibodies during the course of heartwater disease. IgM antibodies reached a maximum on the 4th day after infection and disappeared on the 7th day. IgG antibodies first appeared on the 8th day and continued to increase during the remainder of the observation period of 28 days. The presence of C. ruminantium in the blood fractions of diseased animals was demonstrated by an enzyme-linked immunosorbent assay. The earliest day of C. ruminantium antigen detection was in plasma and serum on the 4th day after inoculation. Of all the blood fractions investigated, the red blood cells showed the highest concentration, and this reached a maximum on the 12th day after infection.

• Authors: A W Neitz, G J Viljoen, J D Bezuidenhout, P T Oberem, L Visser, N M Vermeulen
• Journal: The Onderstepoort journal of veterinary research (Onderstepoort J Vet Res) Vol. 53 Issue 4 Pg. 205-7 (Dec 1986) ISSN: 0030-2465 [Print] South Africa
• PMID: 3796945 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial

Topics

• Animals
• Antibodies, Bacterial (analysis)
• Antigens, Bacterial (analysis)
• Enzyme-Linked Immunosorbent Assay
• Heartwater Disease (immunology)
• Rickettsia (immunology)
• Sheep
• Sheep Diseases (immunology)