Abstract | Objectives: Materials and Methods:
N-succinyl chitosan was synthesized, and the liposomal formulations were prepared and characterized regarding phenolic content assay and morphology. Size, zeta potential, in vitro drug release, and stability. Pulmonary fibrosis was induced by intratracheal bleomycin injection, and hydroxyproline measurements, lung weight, animal body weight, as well as histopathological studies were performed. Results: Succinyl chitosan increases the physical stability of the formulation, especially in acidic conditions. Drug release studies revealed that 66.27% of the loaded drug was released from CF2 in an acidic medium in 2 hr, but 92.31% of the drug was released in 8 hr in a pH=7 medium. An in vivo study demonstrated that rats exposed to bleomycin significantly lost weight, while those treated with CF2 (400 mg/kg) partially regained weight. Bleomycin treatment increased the mean lung weight and the amount of hydroxyproline in the lungs; these values were significantly decreased in the group treated with 400 mg/kg CF2 (P<0.05). Histopathological examination confirmed that treatment with 400 mg/kg CF2 improved lung fibrosis. Conclusion:
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Authors | Neda Bavarsad, Ali Asghar Hemmati, Fateme Jafarian, Azar Mostoufi, Amir Siahpoosh, Mohammadreza Rashidi Nooshabadi, Esrafil Mansouri |
Journal | Iranian journal of basic medical sciences
(Iran J Basic Med Sci)
Vol. 26
Issue 10
Pg. 1237-1244
( 2023)
ISSN: 2008-3866 [Print] Iran |
PMID | 37736512
(Publication Type: Journal Article)
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