Effects of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) on murine and hamster fibroblasts (3T3 and BHK) and murine metastatic melanoma cells (F10).

Abstract

We investigated the action of 6,6'-dithiodinicotinic acid (CPDS) and its metabolite 6-mercaptonicotinic acid (6-MNA) in vitro on murine (3T3) and baby hamster kidney (BHK) fibroblasts and an in vivo highly metastatic subline of murine B16 melanoma (F10). CPDS determined an inhibition of cell growth and a decrease in cell adhesion, while 6-MNA had no effect. When combined with data of the mitotic index and endogenous purine ribonucleotides (on which the drugs seem to have no effect), these observations are conceivable with the hypothesis that the primary target of CPDS is cell membrane.

• Authors: S D'Ancona, G Magnolfi, G Guidetti, G Toffoli, A Lazzarini, M Carrara, M Magon, S Luciani, T Berti
• Journal: Chemioterapia : international journal of the Mediterranean Society of Chemotherapy (Chemioterapia) Vol. 5 Issue 4 Pg. 219-27 (Aug 1986) ISSN: 0392-906X [Print] Italy
• PMID: 3769043 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nicotinic Acids
• Nucleotides
• 6,6'-dithiodinicotinic acid
• 6-mercapto-3-pyridinecarboxylic acid
• Thymidine

Topics

• Cell Adhesion (drug effects)
• Cell Division (drug effects)
• Cell Line
• Dose-Response Relationship, Drug
• Fibroblasts (metabolism, physiology, ultrastructure)
• Kidney Neoplasms (metabolism, ultrastructure)
• Melanoma, Experimental (metabolism, physiopathology, ultrastructure)
• Mitotic Index
• Nicotinic Acids (pharmacology)
• Nucleotides (analysis)
• Thymidine (metabolism)