Study of chromatin structure in ataxia-telangiectasia cells.

Abstract

Micrococcal nuclease was used as a probe to study chromatin structure in control and ataxia-telangiectasia cells. The rate and extent of release of acid-soluble nucleotide was similar in both cell types. Production of mono- and oligonucleosomes by micrococcal nuclease as determined by gel electrophoresis also failed to reveal differences in chromatin structure between control and ataxia-telangiectasia cells. Radiation exposure did not significantly alter the kinetics of digestion. These results indicate that there are no gross alterations in chromatin structure in ataxia-telangiectasia cells.

• Authors: J Houldsworth, S Kumar, M F Lavin
• Journal: Molecular biology reports (Mol Biol Rep) Vol. 11 Issue 3 Pg. 143-7 ( 1986) ISSN: 0301-4851 [Print] Netherlands
• PMID: 3762525 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chromatin
• Nucleosomes
• Micrococcal Nuclease

Topics

• Ataxia Telangiectasia (genetics)
• Cell Nucleus (radiation effects)
• Chromatin (ultrastructure)
• Humans
• Kinetics
• Micrococcal Nuclease (metabolism)
• Nucleosomes (ultrastructure)