Variation of glycinamide ribonucleotide synthetase levels during in vitro aging of human fibroblasts: implications for gene dosage studies.

Abstract

The levels of glycinamide ribonucleotide synthetase were monitored in four human fibroblast cell lines, two diploid, one monosomy 21 and one trisomy 21, from early passage number to senescence. In all four lines, enzyme activity increased as the cells aged. Enzyme levels for the diploid versus trisomy 21 cells did not consistently exhibit the expected gene dosage effect.

• Authors: R G Hards, D Patterson
• Journal: Mechanisms of ageing and development (Mech Ageing Dev) Vol. 36 Issue 1 Pg. 65-70 (Sep 1986) ISSN: 0047-6374 [Print] Ireland
• PMID: 3762234 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Ligases
• Carbon-Nitrogen Ligases
• phosphoribosylamine-glycine ligase

Topics

• Aging
• Carbon-Nitrogen Ligases
• Cell Line
• Diploidy
• Fibroblasts (enzymology, physiology)
• Humans
• Kinetics
• Ligases (metabolism)
• Trisomy