Abstract |
The polysaccharide levan ( polyfructose) has previously been shown to exert an inhibitory effect on the growth of several murine tumors. This activity is mediated by a host reaction, involving mainly macrophages but also other elements of the immune system. It was not clear, however, whether levan-activated macrophages act by a direct cytocidal effect on the tumor cells or via the activation of a specific immune response to the tumor. In the present study, the possibility of a direct cytotoxicity of levan-activated macrophages against Lewis lung carcinoma cells was tested by coculture in vitro. It was found that levan-induced (as well as paraffin oil induced) macrophages actually exert a direct cytotoxic effect on Lewis lung carcinoma cells. The tumor cell killing is mediated by cell to cell contact. A cytoplasmic bridge was often seen between the macrophage and the tumor cell. The remaining tumor cells in the lysed area appear slender, shrunken and non-dividing.
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Authors | J Leibovici, S Hoenig, A Pinchassov |
Journal | International journal of immunopharmacology
(Int J Immunopharmacol)
Vol. 8
Issue 5
Pg. 471-8
( 1986)
ISSN: 0192-0561 [Print] England |
PMID | 3759297
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Cell Communication
- Cytotoxicity, Immunologic
- Fructans
- Lung Neoplasms
(immunology)
- Macrophage Activation
- Male
- Mice
- Mice, Inbred C57BL
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