2-Cyanocinnamic acid sensitization of L929 cells to killing by hyperthermia.

Abstract

Exposure of L929 cells to hyperthermia in the presence of 2-cyanocinnamic acid, an inhibitor of mitochondrial pyruvate transport, markedly enhanced killing at temperatures as low as 41 degrees C. The inhibitor also reduced the oxidation of both glucose and exogenous pyruvate while increasing lactate production from glucose but not from pyruvate. These results are consistent with previous observations that glucose and pyruvate enhance survival after hyperthermia and point to a role for pyruvate in protecting against hyperthermic cytotoxicity. Heat shock protein synthesis was also inhibited by 2-cyanocinnamic acid under some conditions, suggesting that a function of pyruvate may also be involved in heat shock protein induction.

• Authors: H Wang, K W Lanks
• Journal: Cancer research (Cancer Res) Vol. 46 Issue 10 Pg. 5349-52 (Oct 1986) ISSN: 0008-5472 [Print] United States
• PMID: 3756884 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Cinnamates
• Heat-Shock Proteins
• Lactates
• Monocarboxylic Acid Transporters
• Pyruvates
• alpha-cyanocinnamate
• Lactic Acid
• Pyruvic Acid

Topics

• Biological Transport (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Cinnamates (pharmacology)
• Heat-Shock Proteins (biosynthesis)
• Hyperthermia, Induced
• Lactates (metabolism)
• Lactic Acid
• Monocarboxylic Acid Transporters
• Oxidation-Reduction
• Pyruvates (antagonists & inhibitors, metabolism)
• Pyruvic Acid