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Distribution and modulation of surface charges of cells from human leukemia-lymphoma lines at various stages of differentiation.

Abstract
Untreated and retinoic acid (RA) treated human leukemia-lymphoma cell lines reflecting hematopoietic cells at various stages of differentiation, were examined electron microscopically for their surface negative charge distribution using cationized ferritin (CF), an electron dense label of anionic sites. The results indicate that there is a correlation between the CF labeling density/distribution and the stage of lymphoid cell differentiation. Viable unfixed null cell lines show a low CF labeling density with few and small CF patches. A gradual increase in CF labeling density and increase in size and number of CF patches correlates with the stage of differentiation on cell lines of both T or B origin. Treatment of viable unfixed cells with 10(-5) MRA for 10 days seems to prevent the CF-induced formation of CF patches, resulting in a continuous and even distribution of the CF label, similar to that observed on the surface of cells fixed before CF labeling. Some correlation between the distribution of surface anionic sites and the malignant potential of the human leukemic lines could be detected.
AuthorsY Marikovsky, Z Shlomai, O Asher, R Lotan, H Ben-Bassat
JournalCancer (Cancer) Vol. 58 Issue 10 Pg. 2218-23 (Nov 15 1986) ISSN: 0008-543X [Print] United States
PMID3756771 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anions
  • polycationic ferritin
  • Tretinoin
  • Ferritins
Topics
  • Anions
  • Cell Differentiation (drug effects)
  • Cell Line
  • Ferritins
  • Humans
  • Leukemia (physiopathology)
  • Lymphocytes (cytology)
  • Lymphoma (physiopathology)
  • Surface Properties
  • Tretinoin (pharmacology)

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