Nerve growth factor induces changes in (2'-5')oligo(A) synthetase and 2'-phosphodiesterase activities during differentiation of PC12 pheochromocytoma cells.

Abstract

Treatment of rat pheochromocytoma cell line PC12 with Vipera lebetina (snake) nerve growth factor (NGF) induces a rapid increase (from 5 to 25-fold) in the level of (2'-5')oligo(A) synthetase activity and a simultaneous decrease (from 2 to 5-fold) in the activity of 2'-5' A degrading enzymes--2'-phosphodiesterases (2'-PDE). These changes in the enzyme activities led to the significant increase in the intracellular concentration of 2'-5' A. We have found that the serum starvation of PC12 cells causes a 1.5 to 2.0-fold increase in the level of 2'-5' A-synthetase activity, but the activities of 2'-PDE and the intracellular concentration of 2'-5' A remain unaltered. These results show that NGF modulates the activity of (2'-5')oligo(A) enzymes and intracellular concentration of 2'-5' A during the neural differentiation of PC12 cells.

• Authors: M Saarma, U Toots, E Raukas, A Zhelkovsky, A Pivazian, T Neuman
• Journal: Experimental cell research (Exp Cell Res) Vol. 166 Issue 1 Pg. 229-36 (Sep 1986) ISSN: 0014-4827 [Print] United States
• PMID: 3743655 (Publication Type: Journal Article)

Chemical References

• Adenine Nucleotides
• Nerve Growth Factors
• Oligoribonucleotides
• 2',5'-oligoadenylate
• 2',5'-Oligoadenylate Synthetase
• Exoribonucleases
• 2'-phosphodiesterase

Topics

• 2',5'-Oligoadenylate Synthetase (metabolism)
• Adenine Nucleotides (metabolism)
• Animals
• Cell Differentiation
• Cell Line
• Exoribonucleases (metabolism)
• Nerve Growth Factors (pharmacology)
• Neurons (cytology, drug effects, enzymology)
• Oligoribonucleotides (metabolism)
• Pheochromocytoma
• Rats