[Changes in protein kinase activity of the skin in cancer].

Abstract

The phosphorylating enzymes of human skin were studied in bioptic samples of normal tissue (18 samples) and tumors (21 cases), melanomas and basaliomas, which developed in different regions of head and face. The activity of cAMP-dependent and cAMP-independent protein kinases was tested in skin extracts using histone HI and casein as substrates of phosphorylation, respectively. In most tumors the casein kinase activity was found to be significantly elevated (about 10-fold) as compared with normal counterparts. The histone kinase activity was only slightly elevated in tumors (by 30%). For each bioptic sample the ratio of histone kinase activity versus casein kinase activity was calculated. In normal skin this ratio constituted from 1 to 3.7, while in 90% of samples it was higher than 1.5. In all tumors except one the ratio was decreased down to 0.2-0.9. The effect did not depend upon the type of malignancy and tumor location. The change in the protein kinase ratio is considered to be a specific feature of transformed tissue.

• Authors: I D Grozdova, A V Mikhaĭlovskiĭ, I R Eshba, S P Petukhov, V Iu Vasil'ev
• Journal: Voprosy meditsinskoi khimii (Vopr Med Khim) 1986 May-Jun Vol. 32 Issue 3 Pg. 104-8 ISSN: 0042-8809 [Print] Russia (Federation)
• Vernacular Title: Izmenenie aktivnosti proteinkinaz v kozhe pri rake.
• PMID: 3727463 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Protein Kinases

Topics

• Carcinoma, Basal Cell (diagnosis)
• Clinical Enzyme Tests
• Humans
• Melanoma (diagnosis)
• Phosphorylation
• Protein Kinases (metabolism)
• Skin (enzymology)
• Skin Neoplasms (diagnosis)