Abstract | BACKGROUND: Although autologous fat grafting is considered a successful method for the management of contour deformities, the fat graft could potentially induce cancer reappearance by fueling dormant breast cancer cells. Our aim was to characterize the role of adipose-derived stem cells on active and dormant breast cancer cell growth. METHODS: RESULTS: The adipose-derived stem cells showed variable effects on active MCF-7 cells growth and inhibited MCF-7 proliferation after the withdrawal of cobalt chloride. Of the 84 different proteins measured in the conditioned medium, only tenascin-C was differentially expressed in the co-cultures. MCF-7 cells alone did not express tenascin-C, whereas co-cultures between MCF-7 and adipose-derived stem cells expressed more tenascin-C versus adipose-derived stem cells alone. The conditioned medium from co-cultures significantly increased the migration of the cancer cells. CONCLUSIONS: Adipose-derived stem cells themselves neither increased the growth or migration of cancer cells, suggesting that autologous fat grafting may be oncologically safe if reconstruction is postponed until there is no evidence of active disease. However, interactions between adipose-derived stem cells and MCF-7 cancer cells could potentially lead to the production of factors, which further promote cancer cell migration.
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Authors | Maria Nyström, Anne-Therese Lauvrud, Sergio Pérez-Díaz, Paul J Kingham, Rebecca Wiberg |
Journal | Journal of plastic, reconstructive & aesthetic surgery : JPRAS
(J Plast Reconstr Aesthet Surg)
Vol. 83
Pg. 69-76
(08 2023)
ISSN: 1878-0539 [Electronic] Netherlands |
PMID | 37270997
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2023 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
- cobaltous chloride
- Culture Media, Conditioned
- Tenascin
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Topics |
- Humans
- Female
- Adipose Tissue
(transplantation)
- Culture Media, Conditioned
(pharmacology, metabolism)
- Tenascin
(metabolism, pharmacology)
- Breast Neoplasms
- Stem Cells
- Cell Proliferation
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