Abstract |
A clear precipitating arc of factor VII antigen appeared by immunoelectrophoresis using 125I-labelled anti- factor VII antibody incorporated in the Laurell plate to enhance the sensitivity. A plasma sample could be electrophoresed directly on 1% agarose containing 1% rabbit anti-human factor VII antiserum, 0.1% 125I-labelled anti-human factor VII specific IgG and 0.5% polyethyleneglycol 6000, and after washing, autoradiographed at -70 degrees C for 24 hr. A distinct rocket was seen from 45 ul standard reference plasma to 16-fold after auto-radiography. Of 4 patients with congenital factor VII deficiency, 3 patients had no detectable factor VII antigen, one patient had reduced factor VII antigen.
|
Authors | O Takamiya, S Kinoshita, K Yoshioka |
Journal | Thrombosis research
(Thromb Res)
Vol. 42
Issue 6
Pg. 847-53
(Jun 15 1986)
ISSN: 0049-3848 [Print] United States |
PMID | 3726803
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Antigens
(analysis)
- Chemical Precipitation
- Factor VII
(analysis, immunology)
- Factor VII Deficiency
(immunology)
- Humans
- Immunoelectrophoresis
- Radioimmunoassay
(methods)
|