miR-125b-5p, miR-155-3p, and miR-214-5p and Target E2F2 Gene in Oral Squamous Cell Carcinoma.

Abstract

It is known that E2F2 (E2F transcription factor 2) plays an important role as controller in the cell cycle. This study aimed to analyse the expression of the E2F2 gene and E2F2 protein and demonstrate E2F2 target microRNAs (miRNAs) candidates (miR-125b-5p, miR-155-3p, and miR-214-5p) in oral squamous cell carcinoma tumour and margin samples. The study group consisted 50 patients. The E2F2 gene and miRNAs expression levels were assessed by qPCR, while the E2F2 protein was assessed by ELISA. When analysing the effect of miRNAs expression on E2F2 gene expression and E2F2 protein level, we observed no statistically significant correlations. miR-125b-5p was downregulated, while miR-155-3p, and miR-214-5p were upregulated in tumour samples compared to margin. We observed a difference between the miR-125b-5p expression level in smokers and non-smokers in margin samples. Furthermore, HPV-positive individuals had a significantly higher miR-125b-5p and miR-214-5p expression level compared to HPV-negative patients in tumour samples. The study result showed that the E2F2 gene is not the target for analysed miRNAs in OSCC. Moreover, miR-155-3p and miR-125b-5p could play roles in the pathogenesis of OSCC. A differential expression of the analysed miRNAs was observed in response to tobacco smoke and HPV status.

• Authors: Karolina Gołąbek, Dorota Hudy, Agata Świętek, Jadwiga Gaździcka, Natalia Dąbrowska, Katarzyna Miśkiewicz-Orczyk, Natalia Zięba, Maciej Misiołek, Joanna Katarzyna Strzelczyk
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 24 Issue 7 (Mar 28 2023) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 37047293 (Publication Type: Journal Article)

Chemical References

• E2F2 Transcription Factor
• MicroRNAs
• E2F2 protein, human
• MIRN155 microRNA, human
• MIRN214 microRNA, human

Topics

• Humans
• Carcinoma, Squamous Cell (genetics)
• Squamous Cell Carcinoma of Head and Neck (genetics)
• E2F2 Transcription Factor (genetics, metabolism)
• Papillomavirus Infections (genetics)
• Mouth Neoplasms (genetics)
• MicroRNAs (genetics, metabolism)
• Head and Neck Neoplasms (genetics)
• Gene Expression Regulation, Neoplastic