Fibrinopeptide A and the phosphate content of fibrinogen in venous thromboembolism and disseminated intravascular coagulation.

Concentrations of plasma fibrinopeptide A (FPA) were measured by radioimmunoassay in 50 patients with venous thromboembolism or disseminated intravascular coagulation or both. A consistent discrepancy was observed in values obtained with two anti-FPA antisera. Analysis of extracts from plasma of these patients by high-performance liquid chromatography (HPLC) revealed the presence of a phosphorylated and an unphosphorylated form of the A peptide. Differences in concentrations of FPA measured with the two antisera could be accounted for by their different reactivity with phosphorylated FPA (FPA-P). The differences were abolished by treatment with alkaline phosphatase. A good correlation was observed between the FPA-P content of free A-peptide material and of fibrinogen in plasma as determined by HPLC (r = .88, P less than .001, n = 11). In patients with elevated FPA levels, the mean FPA-P content of fibrinogen was significantly higher (P less than .002, n = 13) than in patients with normal FPA levels (n = 8) and in healthy controls (n = 14). Phosphorus in fibrinogen did not correlate with fibrinogen degradation products or fibrinogen levels and became normal on adequate anticoagulation. Therefore, blood-clotting activation may lead to a high phosphate content of fibrinogen and of free FPA in plasma.
AuthorsO C Leeksma, F Meijer-Huizinga, E A Stoepman-van Dalen, C J van Ginkel, W G van Aken, J A van Mourik
JournalBlood (Blood) Vol. 67 Issue 5 Pg. 1460-7 (May 1986) ISSN: 0006-4971 [Print] UNITED STATES
PMID3697510 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Phosphates
  • Fibrinopeptide A
  • Fibrin
  • Fibrinogen
  • Heparin
  • Adult
  • Aged
  • Chromatography, High Pressure Liquid
  • Disseminated Intravascular Coagulation (blood, drug therapy)
  • Female
  • Fibrin (analysis)
  • Fibrinogen (analysis)
  • Fibrinopeptide A (analysis)
  • Heparin (therapeutic use)
  • Humans
  • Male
  • Middle Aged
  • Phlebitis (blood, drug therapy)
  • Phosphates (analysis)

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