HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Fibrinopeptide A and the phosphate content of fibrinogen in venous thromboembolism and disseminated intravascular coagulation.

Abstract
Concentrations of plasma fibrinopeptide A (FPA) were measured by radioimmunoassay in 50 patients with venous thromboembolism or disseminated intravascular coagulation or both. A consistent discrepancy was observed in values obtained with two anti-FPA antisera. Analysis of extracts from plasma of these patients by high-performance liquid chromatography (HPLC) revealed the presence of a phosphorylated and an unphosphorylated form of the A peptide. Differences in concentrations of FPA measured with the two antisera could be accounted for by their different reactivity with phosphorylated FPA (FPA-P). The differences were abolished by treatment with alkaline phosphatase. A good correlation was observed between the FPA-P content of free A-peptide material and of fibrinogen in plasma as determined by HPLC (r = .88, P less than .001, n = 11). In patients with elevated FPA levels, the mean FPA-P content of fibrinogen was significantly higher (P less than .002, n = 13) than in patients with normal FPA levels (n = 8) and in healthy controls (n = 14). Phosphorus in fibrinogen did not correlate with fibrinogen degradation products or fibrinogen levels and became normal on adequate anticoagulation. Therefore, blood-clotting activation may lead to a high phosphate content of fibrinogen and of free FPA in plasma.
AuthorsO C Leeksma, F Meijer-Huizinga, E A Stoepman-van Dalen, C J van Ginkel, W G van Aken, J A van Mourik
JournalBlood (Blood) Vol. 67 Issue 5 Pg. 1460-7 (May 1986) ISSN: 0006-4971 [Print] UNITED STATES
PMID3697510 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Phosphates
  • Fibrinopeptide A
  • Fibrin
  • Fibrinogen
  • Heparin
Topics
  • Adult
  • Aged
  • Chromatography, High Pressure Liquid
  • Disseminated Intravascular Coagulation (blood, drug therapy)
  • Female
  • Fibrin (analysis)
  • Fibrinogen (analysis)
  • Fibrinopeptide A (analysis)
  • Heparin (therapeutic use)
  • Humans
  • Male
  • Middle Aged
  • Phlebitis (blood, drug therapy)
  • Phosphates (analysis)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: