The acute and sub-acute toxicity studies were performed in male and female Swiss albino mice as per the guidelines mentioned in OECD. The
oral administration of M. tridentata stem extract (MSE) showed no treatment-related mortality and
body weight change in mice up to the single dose of 30,000 mg/kg
body weight in the acute toxicity study and up to a dose of 30,000 mg/kg/day
body weight in the sub-acute toxicity study. Moreover, the clinical signs,
body weight, gross pathology, organ weight, hematology (except for platelet count), biochemical analysis, and histopathology did not show significant variation at a medium dose of 15,000 mg/kg/day
body weight compared to the control group. However, toxicological signs in behavior, very mild
interstitial nephritis, as well as significant variation in platelet count and total
protein parameters were observed at a dose of 30,000 mg/kg/day in the 28-day oral toxicity study. Thus, the no-observed-adverse-effect level was determined at a dose of 15,000 mg/kg/day
body weight. Based on study results, it is concluded that MSE showed LD50 of greater than 5000 mg/kg/day
body weight. Hence, this could be a potential candidature as a future safe
pharmaceutical product.