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Characterization of a novel LTA/LPS-binding antimicrobial and anti-inflammatory temporin peptide from the skin of Fejervary limnocharis (Anura: Ranidae).

Abstract
Septic shock caused by Gram-positive bacteria continues to be a major cause of morbidity and mortality in intensive care units globally. Most Temporins are excellent growth inhibitors of gram-positive bacteria and candidates for developing antimicrobial treatments due to their biological action and small molecular weight. In this study, a novel Temporin peptide from the skin of Fejervarya limnocharis frog, named as Temporin-FL, was characterized. Temporin-FL was found to adopt typical α-helical conformation in SDS solution and to exhibit selective antibacterial activity against Gram-positive bacteria through a membrane destruction mechanism. Accordingly, Temporin-FL showed protective effects against Staphylococcus aureus-induced sepsis in mice. Finally, Temporin-FL was demonstrated to exert anti-inflammatory effects by neutralizing the action of LPS/LTA and by inhibiting MAPK pathway activation. Therefore, Temporin-FL represents a novel candidate for moleculartherapy of Gram-positive bacterial sepsis.
AuthorsJinqiao Li, Yan Liang, Minhong Su, Jiena Wu, Jinwei Chai, Weichen Xiong, Guoxiang Mo, Xin Chen, Xueqing Xu
JournalBiochemical pharmacology (Biochem Pharmacol) Vol. 210 Pg. 115471 (04 2023) ISSN: 1873-2968 [Electronic] England
PMID36893813 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2023 Elsevier Inc. All rights reserved.
Chemical References
  • temporin
  • Lipopolysaccharides
  • Amphibian Proteins
  • Antimicrobial Cationic Peptides
  • Anti-Infective Agents
  • Anti-Bacterial Agents
Topics
  • Animals
  • Mice
  • Lipopolysaccharides (toxicity)
  • Amino Acid Sequence
  • Amphibian Proteins (pharmacology, therapeutic use, chemistry)
  • Antimicrobial Cationic Peptides (pharmacology, therapeutic use)
  • Anti-Infective Agents (pharmacology, therapeutic use, chemistry)
  • Anti-Bacterial Agents (pharmacology, therapeutic use, metabolism)
  • Ranidae (metabolism)
  • Skin
  • Gram-Positive Bacteria
  • Shock, Septic (metabolism)
  • Microbial Sensitivity Tests

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