Abstract |
Septic shock caused by Gram-positive bacteria continues to be a major cause of morbidity and mortality in intensive care units globally. Most Temporins are excellent growth inhibitors of gram-positive bacteria and candidates for developing antimicrobial treatments due to their biological action and small molecular weight. In this study, a novel Temporin peptide from the skin of Fejervarya limnocharis frog, named as Temporin-FL, was characterized. Temporin-FL was found to adopt typical α-helical conformation in SDS solution and to exhibit selective antibacterial activity against Gram-positive bacteria through a membrane destruction mechanism. Accordingly, Temporin-FL showed protective effects against Staphylococcus aureus-induced sepsis in mice. Finally, Temporin-FL was demonstrated to exert anti-inflammatory effects by neutralizing the action of LPS/LTA and by inhibiting MAPK pathway activation. Therefore, Temporin-FL represents a novel candidate for moleculartherapy of Gram-positive bacterial sepsis.
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Authors | Jinqiao Li, Yan Liang, Minhong Su, Jiena Wu, Jinwei Chai, Weichen Xiong, Guoxiang Mo, Xin Chen, Xueqing Xu |
Journal | Biochemical pharmacology
(Biochem Pharmacol)
Vol. 210
Pg. 115471
(04 2023)
ISSN: 1873-2968 [Electronic] England |
PMID | 36893813
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2023 Elsevier Inc. All rights reserved. |
Chemical References |
- temporin
- Lipopolysaccharides
- Amphibian Proteins
- Antimicrobial Cationic Peptides
- Anti-Infective Agents
- Anti-Bacterial Agents
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Topics |
- Animals
- Mice
- Lipopolysaccharides
(toxicity)
- Amino Acid Sequence
- Amphibian Proteins
(pharmacology, therapeutic use, chemistry)
- Antimicrobial Cationic Peptides
(pharmacology, therapeutic use)
- Anti-Infective Agents
(pharmacology, therapeutic use, chemistry)
- Anti-Bacterial Agents
(pharmacology, therapeutic use, metabolism)
- Ranidae
(metabolism)
- Skin
- Gram-Positive Bacteria
- Shock, Septic
(metabolism)
- Microbial Sensitivity Tests
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