Biostimulative effects of Nd:YAG Q-switch dye on normal human fibroblast cultures: study of a new chemosensitizing agent for the Nd:YAG laser.

Abstract

Kodak Q-switch II is a new chemical with an absorption maxima at 1,051 nm, designed to be used as an Nd:YAG dye laser. The potential for this dye as a new chemosensitizing agent in the treatment of connective tissue diseases and wound healing with low energy Nd:YAG laser was examined. Two normal fibroblast cell lines were tested for sensitivity to various levels of this dye in vitro. These cells were exposed to Q-switch II dye at concentrations of 0.01, 0.1, 1, 10, 50, and 100 micrograms/ml for 1 and 24 hours. Cell viability was assessed by the trypan blue exclusion test. Cell duplication and DNA synthesis were measured by the incorporation of [3H]-thymidine at 6 and 24 hours postexposure to Q-switch II dye. At concentrations up to 10 micrograms/ml, both cell lines tested showed no changes in cell viability. However, at concentrations equal or higher than 50 micrograms/ml, more than 40% of the fibroblasts incorporated trypan blue after 24 hours of exposure to this dye, indicating significant cell destruction. The results indicate that Q-switch II dye is nontoxic to normal human fibroblast cultures and showed significant biostimulative effects on cell duplication at concentrations equal to or lower than 10 micrograms/ml. Further studies will be required to determine the usefulness of Q-switch II dye as a new photochemosensitizing agent for potential biostimulation of wound healing and/or treatment of connective tissue diseases with the Nd:YAG laser (near infrared, 1,060 nm) at "nonthermal" levels of energies.

• Authors: D J Castro, R E Saxton, H R Fetterman, D J Castro, P H Ward
• Journal: The Laryngoscope (Laryngoscope) Vol. 97 Issue 12 Pg. 1454-9 (Dec 1987) ISSN: 0023-852X [Print] United States
• PMID: 3683057 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Benzopyrans
• Radiation-Sensitizing Agents
• Q-switch II dye
• DNA

Topics

• Benzopyrans (pharmacology)
• Cell Line
• Cell Survival (drug effects)
• Chemical Phenomena
• Chemistry
• DNA (biosynthesis)
• Fibroblasts (cytology, drug effects, metabolism)
• Humans
• Laser Therapy
• Osmolar Concentration
• Radiation-Sensitizing Agents (pharmacology)
• Skin (cytology, drug effects, metabolism)
• Time Factors