Endogenous polymers of ADP-ribose are associated with the nuclear matrix.

Abstract

The metabolism of nuclear polymers of ADP-ribose has been implicated in several chromatin-associated processes. However, the distribution of endogenous ADP-ribose polymers in the nucleus or within different fractions of chromatin has not been studied. Using a procedure which allowed the radiolabeling and detection of endogenous polymers of ADP-ribose, we have analyzed the nuclear distribution of these polymers in untreated cells and in cells subjected to hyperthermia, N-methyl-N'-nitro-N-nitrosoguanidine, or both. When isolated nuclei from cells subjected to any of these conditions were digested with micrococcal nuclease such that 80% of the DNA was released, 90% of the total poly(ADP-ribose) remained with the micrococcal nuclease resistant chromatin fraction. When nuclear matrix fractions were prepared by exhaustive DNase I digestion in combination with three different salt extraction procedures (2 M NaCl, 300 mM (NH4)2SO4 or 25 mM lithium diiodosalicylate), the matrices contained less than 1% of the total nuclear DNA but 50 to 70% of the total poly(ADP-ribose). These data suggest that the nuclear matrix may be a major site of poly(ADP-ribose) metabolism.

• Authors: M E Cardenas-Corona, E L Jacobson, M K Jacobson
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 262 Issue 31 Pg. 14863-6 (Nov 05 1987) ISSN: 0021-9258 [Print] United States
• PMID: 3667608 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Nucleoside Diphosphate Sugars
• Poly Adenosine Diphosphate Ribose
• DNA

Topics

• Animals
• Cell Fractionation
• Cell Nucleus (metabolism, ultrastructure)
• Cells, Cultured
• DNA (metabolism)
• Kinetics
• Mice
• Nucleoside Diphosphate Sugars (metabolism)
• Poly Adenosine Diphosphate Ribose (metabolism)