Localization of the human dihydropteridine reductase gene to band p15.3 of chromosome 4 by in situ hybridization.

Abstract

We report the localization of the gene for dihydropteridine reductase (DHPR) to the human chromosome region 4p15.3 by in situ hybridization using a cDNA probe to the enzyme. The distal end of the short arm of chromosome 4 is of considerable interest because the gene responsible for Huntington's disease is located in this region. Although this part of the chromosome is being extensively studied, DHPR is the first well-characterised gene to be assigned to the region. Restriction enzyme fragment length polymorphisms have been detected with a number of restriction endonucleases, including AvaII and MspI. These features may make the DHPR cDNA clone a useful probe not only for prenatal diagnosis of DHPR deficiency but also for linkage studies of Huntington's disease.

• Authors: R M Brown, H H Dahl
• Journal: Genomics (Genomics) Vol. 1 Issue 1 Pg. 67-70 (Sep 1987) ISSN: 0888-7543 [Print] United States
• PMID: 3666748 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA
• Dihydropteridine Reductase
• NADH, NADPH Oxidoreductases

Topics

• Base Sequence
• Chromosome Banding
• Chromosome Mapping
• Chromosomes, Human, Pair 4
• DNA (genetics)
• Dihydropteridine Reductase (genetics)
• Humans
• Karyotyping
• Molecular Sequence Data
• NADH, NADPH Oxidoreductases (genetics)
• Nucleic Acid Hybridization