An antiproliferative salicylaldoximato type copper(II) chelate as an enhancer of the cytotoxicity of murine spleen cells to tumor cells in vitro.

Abstract

The antiproliferative metal chelate bis(2,3,4-trihydroxybenzaldoxyimato)copper(II) (CuTRI2) was shown to distinctly enhance the cytotoxicity of the spleen effector cells of CBA/CA mice to YAC-1 tumor cells in vitro as measured using a 4-h 51Cr release test. The compound was either added directly to the assay system or the effectors were incubated in the presence of it for 2 h and washed before assay. The concentrations tested were 10, 20 and 40 mg/ml, the lowest concentration leading invariably to the strongest enhancement of cytotoxicity. The effector cells involved were very probably natural killer cells. The highest concentration had, if anything, a negative effect on the release of 51Cr from the target cells. In the same concentrations, CuTRI2 did not enhance the cytotoxicity of the spleen effector cells to K-562 tumor cells. Preliminary studies on the related chelate trans-bis(2,4-dihydroxybenzaldoximato)copper(II) are also reported. This paper is apparently the first report of a copper chelate that enhances the cytotoxicity of any sort of effector cells to tumor cells.

• Authors: H Elo
• Journal: Cancer letters (Cancer Lett) Vol. 36 Issue 3 Pg. 333-9 (Sep 1987) ISSN: 0304-3835 [Print] Ireland
• PMID: 3652032 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chelating Agents
• Chromium Radioisotopes
• Organometallic Compounds
• bis(2,3,4-trihydroxybenzaldoxyimato)copper(II)
• bis(resorcylaldoximato)copper(II)

Topics

• Animals
• Cell Division (drug effects)
• Cells, Cultured
• Chelating Agents (pharmacology)
• Chromium Radioisotopes
• Cytotoxicity Tests, Immunologic
• Cytotoxicity, Immunologic (drug effects)
• Humans
• Killer Cells, Natural (drug effects, immunology)
• Mice
• Organometallic Compounds (pharmacology)