Properties and prenatal ontogeny of beta-D-mannosidase in selected goat tissues.

Abstract

beta-D-Mannosidase activity in selected normal adult, neonatal and foetal goat tissues and in tissues from animals affected with caprine beta-mannosidosis was examined with the use of 4-methylumbelliferyl beta-D-mannopyranoside as substrate. The enzyme in normal adult thyroid, kidney and brain exhibited a sharp unimodal pH optimum at pH 5.0, whereas the enzyme in both normal adult and mutant liver exhibited broad pH ranges of activity (pH 4.5-8.0). No residual enzyme was detectable in mutant kidney or brain; in contrast, residual activity in mutant liver was 52% of that in a neonatal control. Concanavalin A-Sepharose 4B (Con A-Sepharose) fractionation of normal adult liver beta-D-mannosidase resolved the enzyme into an unbound (non-lysosomal) from (52%) with a broad pH range of activity (pH 4.5-8.0) and a bound (lysosomal) form (48%) with a sharp pH optimum of 5.5. The enzyme in mutant liver consisted entirely of the unbound (non-lysosomal) form. Beta-D-Mannosidase activity in normal adult thyroid, kidney and brain was resolved by chromatofocusing into two major isoenzymes, with pI 5.5 and 5.9, and traces of a minor isoenzyme, with pI 5.0. In normal adult liver the enzyme was also resolved into three isoenzymes with similar pI values; however, that with pI 5.0 predominated. The predominant form of the enzyme in 60-day-foetal liver was bound by Con A, exhibited a unimodal pH optimum (5.0) and was resolved into two isoenzymes, with pI 5.4 and 5.8; only traces of an isoenzyme with pI 5.0 were detectable. Total hepatic beta-D-mannosidase activity increased progressively towards adult values during the last 90 days of gestation as a result of increasing non-lysosomal isoenzyme activity (pI 5.0). Lysosomal beta-D-mannosidase was shown to occur in all normal goat tissues studied as multiple isoenzymes, which are genetically and developmentally distinct from the non-lysosomal isoenzyme occurring predominantly, if not exclusively, in liver.

• Authors: R D Pearce, J W Callahan, P B Little, D T Armstrong, D Kiehm, J T Clarke
• Journal: The Biochemical journal (Biochem J) Vol. 243 Issue 2 Pg. 603-9 (Apr 15 1987) ISSN: 0264-6021 [Print] England
• PMID: 3632638 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Mannosidases
• beta-Mannosidase

Topics

• Animals
• Animals, Newborn (metabolism)
• Electrophoresis, Cellulose Acetate
• Fetus (enzymology)
• Goats (embryology, metabolism)
• Hydrogen-Ion Concentration
• Isoelectric Focusing
• Liver (embryology, enzymology)
• Mannosidases (metabolism)
• Tissue Distribution
• beta-Mannosidase