NH2-terminal of gastrin-17 in duodenal ulcer disease: identification of progastrin-17.

Abstract

Serum gastrin concentrations were measured using antisera with specificity for the carboxyl and amino terminus of gastrin-17 in 50 healthy subjects and 18 patients with active duodenal ulcer disease (DU). The amino terminal of gastrin-17 immunoreactivity was significantly higher in DU patients than in healthy subjects. NH2-terminus of gastrin-17 immunopurified material from serum of DU patients was subjected to Sephadex G50 column chromatography and eluates were monitored by an additional antiserum EG10 that recognizes COOH-terminally extended gastrin. Besides the NH2 terminal tridecapeptide of gastrin-17, COOH-terminally extended progastrin was found. This may reflect abnormal processing of gastrin in patients with active duodenal ulcer disease.

• Authors: P C Kothary, A I Vinik
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 146 Issue 2 Pg. 884-8 (Jul 31 1987) ISSN: 0006-291X [Print] United States
• PMID: 3619907 (Publication Type: Journal Article)

Chemical References

• Gastrins
• Immune Sera
• Protein Precursors
• big gastrin
• gastrin 17

Topics

• Adolescent
• Adult
• Aged
• Amino Acid Sequence
• Duodenal Ulcer (blood)
• Female
• Gastrins (analysis, blood)
• Humans
• Immune Sera
• Male
• Middle Aged
• Protein Precursors (analysis)