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A simple method for maintaining relative positions of separate tissue elements during processing for electron microscopy.

Abstract
Molten (328 K) 20% gelatin is used as a 'glue' to hold together separate tissue elements or tissue elements that may be separated when cutting small blocks of tissue for plastic embedding. Standard aldehyde and osmium fixation, dehydration and epoxy embedding are compatible with this as is semi-thin sectioning for light microscopy or thin sectioning for electron microscopy.
AuthorsC A Stirling
JournalJournal of microscopy (J Microsc) Vol. 114 Issue 1 Pg. 107-9 (Sep 1978) ISSN: 0022-2720 [Print] England
PMID361961 (Publication Type: Journal Article)
Chemical References
  • Gelatin
Topics
  • Animals
  • Gelatin
  • Histological Techniques
  • Mice
  • Microscopy, Electron
  • Muscular Dystrophy, Animal (pathology)
  • Pia Mater (ultrastructure)
  • Spinal Nerve Roots (pathology)

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