The cytotoxicity of karanjin toward breast cancer cells is involved in the PI3K/Akt signaling pathway.

Abstract

Karanjin is a bioactive furanoflavonoid with various pharmacological activities including anticancer activities. However, the effect and the related mechanism of karanjin in breast cancer (BC) have not been revealed. The potential targets of karanjin and BC were predicted using SwissTargetPrediction and GeneCards databases, respectively. The overlapping targets between karanjin and BC were identified using the Venn diagram. DAVID database was used for the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analysis. Cell viability, proliferation, and apoptosis were examined by MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-tetrazolium bromide), EdU (5-ethynyl-2'-deoxyuridine) incorporation, and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP digoxigenin nick-end labeling) assays, respectively. The protein levels were measured by western blot analysis. We screened out 28 overlapping targets between karanjin and BC. KEGG analysis showed that the targets of karanjin in BC were associated with the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway. Karanjin inhibited cell viability and impeded the proliferative ability of BC cells. Moreover, karanjin treatment induced apoptosis in BC cells. Additionally, karanjin treatment blocked the PI3K/Akt signaling pathway and activation of the PI3K/Akt pathway reversed the antitumor effect of karanjin on BC cells. In conclusion, karanjin exerted antitumor activity in BC cells by regulating the PI3K/Akt signaling pathway.

• Authors: Jinsong Yu, Han Yang, Chunliu Lv, Xiaowei Dai
• Journal: Drug development research (Drug Dev Res) Vol. 83 Issue 7 Pg. 1673-1682 (11 2022) ISSN: 1098-2299 [Electronic] United States
• PMID: 36065628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 Wiley Periodicals LLC.

Chemical References

• Proto-Oncogene Proteins c-akt
• Phosphatidylinositol 3-Kinase
• Phosphatidylinositol 3-Kinases
• karanjin

Topics

• Humans
• Female
• Proto-Oncogene Proteins c-akt (metabolism)
• Phosphatidylinositol 3-Kinase (metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Breast Neoplasms (drug therapy, pathology)
• Signal Transduction
• Apoptosis
• Cell Proliferation