Normothermic Ex Vivo Pancreas Perfusion for the Preservation of Pancreas Allografts before Transplantation.

Abstract

Pancreas transplantation (PTx) is a curative treatment for people who live with the burden of a diagnosis of diabetes mellitus (DM). However, due to organ shortages and increasing numbers of patients being listed for PTx, new strategies are needed to increase the number of available grafts for transplantation. Static cold storage (SCS) is considered the gold standard for standard criteria organs. However, standard criteria donors (SCD) are becoming scarce and new strategies that can increase the rate of organ acceptance from extended criteria donors (ECD) are urgently needed. Normothermic ex vivo perfusion (NEVP) is one of the strategies that has become increasingly popular over the past couple of decades. This preservation method has already been used successfully in other organs (liver, kidneys, and lungs) but has been minimally explored in pancreas transplantation. The few papers that describe the method for pancreas show little success, edema being one of the major issues. The following manuscript describes the successful NEVP method and setup developed by our group to perfuse swine pancreas.

• Authors: Catherine Parmentier, Samrat Ray, Laura Mazilescu, Masataka Kawamura, Yuki Noguchi, Emmanuel Nogueira, Sujani Ganesh, Bhranavi Arulratnam, Sangeetha Kalimuthu, Markus Selzner, Trevor Reichman
• Journal: Journal of visualized experiments : JoVE (J Vis Exp) Issue 185 (07 27 2022) ISSN: 1940-087X [Electronic] United States
• PMID: 35969078 (Publication Type: Journal Article, Video-Audio Media)

Topics

• Allografts
• Humans
• Organ Preservation (methods)
• Pancreas (surgery)
• Perfusion (methods)
• Tissue Donors