Cerebral metabolism in brain tumor of mice studied by in vivo 31P-NMR spectroscopy.

Abstract

We now report a mouse model system of brain tumor for 31P-NMR spectroscopic study of in vivo cerebral metabolism. In vivo 31P-NMR (109 MHz) spectra were taken on the 9th day by the Faraday shield method of the brain of mice (3-week-old) transplanted intracerebrally with mKS X A tumor cells. In tumor-bearing mice, the amount of creatine phosphate decreased markedly and that of inorganic phosphate plus sugar phosphate increased accordingly. Furthermore, the broadening and splitting of individual signals were also noted with tumor-bearing mice; this is interpreted as indicating a variety of changes in chemical shift occurring in the brain of the animals due to heterogeneous distribution of pH. Binding or detaching of divalent cations to and from phosphometabolites may also be responsible for these changes.

• Authors: K Kato, A Ohsaka, K Matsushita, K Yoshikawa
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 144 Issue 1 Pg. 53-8 (Apr 14 1987) ISSN: 0006-291X [Print] United States
• PMID: 3579925 (Publication Type: Journal Article)

Chemical References

• Phosphocreatine
• Phosphorus
• Adenosine Triphosphate
• Magnesium

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Brain (metabolism)
• Brain Neoplasms (metabolism)
• Hydrogen-Ion Concentration
• Magnesium (metabolism)
• Magnetic Resonance Spectroscopy
• Mice
• Mice, Inbred BALB C
• Phosphocreatine (metabolism)
• Phosphorus