A novel long excitation/emission wavelength fluorophore as platform utilized to construct NIR probes for bioimaging and biosensing.

Abstract

Near-infrared (NIR) fluorophores, especially dicyano-based fluorophores and xanthene-based hemicyanines, have beenput high expectation in bioimaging application due to their excellent optical properties. However, they suffer from inherentshortagessuch as short excitation/emission wavelength (less than 700 nm) or small Stokes shift (20-50 nm). Herein, we constructed a novel NIR dicyano-based fluorophore (DCO-HBTN). Toourknowledge, it is the first reported dicyano-based fluorophore of which the excitation/emission wavelength is more than 650 nm and Stokes shift is more than 100 nm. To demonstrate the feasibility of our efforts, we developed two NIR fluorescent probes (Probe-Cys and Probe-H2S) based on the fluorophore, Probe-Cys displayed good selective and highly sensitive (LOD = 0.28 μM) recognition of Cys over Hcy and GSH, which was used to visualize endogenous Cys in tumor tissue. Probe-H2S exhibited an. excellent specific and sensitive (LOD = 0.11 μM) response to H2S, which was applied in monitoring H2S releasing from the prodrug in vitro and in vivo.

• Authors: Jingcan Qin, Hao Tian, Fei Kong, Qian Qian Zhao, Chuan Zhang, Hongmei Gu, Yuehua Li
• Journal: Bioorganic chemistry (Bioorg Chem) Vol. 127 Pg. 105954 (10 2022) ISSN: 1090-2120 [Electronic] United States
• PMID: 35753118 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Chemical References

• Fluorescent Dyes
• Xanthenes
• Cysteine

Topics

• Cysteine
• Fluorescent Dyes
• HeLa Cells
• Humans
• Xanthenes