Golgi phosphoprotein 3 promotes ovarian cancer progression and is associated with cisplatin resistance.

Abstract

Background:

Golgi phosphoprotein-3 (GOLPH 3) is involved in the development of several human cancers. However, the clinical significance and biological role of GOLPH 3 in ovarian cancer (OC) remains unknown.

Methods:

The expression of GOLPH 3 in OC cell lines was quantified using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assays. The role of GOLPH 3 in tumorigenicity, migration, and invasion of OC cell lines by small interference RNA, scratch wound-healing assays, and transwell assays was detected. In addition, western blotting was used to determine whether GOLPH 3 is associated with the PI3K/AKT/mTOR signaling pathway. Furthermore, RT-qPCR verified whether GOLPH 3 is associated with drug resistance.

Results:

GOLPH 3-positive expression rate was higher in OC. Downregulation of GOLPH 3 markedly inhibited the migration and invasion and may be related to the PI3K/AKT/mTOR signal pathway. Moreover, the result of the experiment proved that GOLPH 3 enhances the sensitivity of OC to cisplatin by regulating ATP7A/B. GOLPH 3 promoted the invasion and migration of OC, and the mechanism may be related to the PI3K/Akt/mTOR pathway. In addition, inhibition of GOLPH 3 increased the sensitivity of OC cells to cisplatin, which may be associated with ATP7A/B.

Conclusion:

This study found that GOLPH3 may promote the migration and invasion of OC cells through PI3K/Akt/mTOR pathway. At the same time, low expression of GOLPH3 increased the sensitivity of OC cells to cisplatin.

• Authors: Teng Liu, Zhen-Wei Jin, Ying Li, Ge Zhang, Xiao-Ying Yang, Xiao-Meng Xu, Ying-Chun Ma
• Journal: Journal of cancer research and therapeutics (J Cancer Res Ther) Vol. 18 Issue 2 Pg. 488-495 (Apr 2022) ISSN: 1998-4138 [Electronic] India
• PMID: 35645119 (Publication Type: Journal Article)

Chemical References

• GOLPH3 protein, human
• Membrane Proteins
• Phosphoproteins
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• Cisplatin

Topics

• Carcinoma, Ovarian Epithelial
• Cell Movement (genetics)
• Cell Proliferation
• Cisplatin (pharmacology)
• Drug Resistance, Neoplasm
• Female
• Humans
• Membrane Proteins (genetics)
• Ovarian Neoplasms (drug therapy, genetics, metabolism)
• Phosphatidylinositol 3-Kinases (genetics, metabolism)
• Phosphoproteins
• Proto-Oncogene Proteins c-akt (metabolism)
• TOR Serine-Threonine Kinases (genetics)