Abstract |
Promyelocytic leukemia (PML) proteins are involved in the pathogenesis of acute promyelocytic leukemia (APL). Trivalent arsenic (As3+) is known to cure APL by binding to cysteine residues of PML and enhance the degradation of PML- retinoic acid receptor α (RARα), a t(15;17) gene translocation product in APL cells, and restore PML-nuclear bodies (NBs). The size, number, and shape of PML-NBs vary among cell types and during cell division. However, topological changes of PML-NBs in As3+-exposed cells have not been well-documented. We report that As3+-induced solubility shift underlies rapid SUMOylation of PML and late agglomeration of PML-NBs. Most PML-NBs were toroidal and granular dot-like in GFPPML-transduced CHO-K1 and HEK293 cells, respectively. Exposure to As3+ and antimony (Sb3+) greatly reduced the solubility of PML and enhanced SUMOylation within 2 h in the absence of changes in the number and size of PML-NBs. However, the prolonged exposure to As3+ and Sb3+ resulted in agglomeration of PML-NBs. Exposure to bismuth (Bi3+), another Group 15 element, did not induce any of these changes. ML792, a SUMO activation inhibitor, reduced the number of PML-NBs and increased the size of the NBs, but had little effect on the As3+-induced solubility change of PML. These results warrant the importance of As3+- or Sb3+-induced solubility shift of PML for the regulation intranuclear dynamics of PML-NBs.
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Authors | Seishiro Hirano, Osamu Udagawa |
Journal | PloS one
(PLoS One)
Vol. 17
Issue 5
Pg. e0268835
( 2022)
ISSN: 1932-6203 [Electronic] United States |
PMID | 35594310
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Promyelocytic Leukemia Protein
- Transcription Factors
- Arsenic
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Topics |
- Arsenic
(metabolism, pharmacology)
- Cell Nucleus
(metabolism)
- HEK293 Cells
- Humans
- Leukemia, Promyelocytic, Acute
(metabolism)
- Nuclear Bodies
(chemistry)
- Promyelocytic Leukemia Protein
(genetics, metabolism)
- Solubility
- Transcription Factors
(metabolism)
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