Abstract |
With the use of a percoll gradient separation procedure, eosinophils of individuals with asthma and with allergy could be separated into normal- and low-density cell fractions. The presence of low-density eosinophils possibly reflects an ongoing process of activation of these cells induced by the allergic reaction. Ca- ionophore-induced leukotriene (LT) C4 production, in the absence of added substrates, demonstrated a decreased potency for LT generation by low-density eosinophils compared with the LT generation of normal-density cells (57 +/- 33 ng and 103 +/- 44 ng per 10(6) cells, respectively). In contrast with the Ca- ionophore-induced LT formation, incubations with serum-treated zymosan in the presence of glutathione demonstrated higher productions of LTC4 with the low-density eosinophilic subpopulation compared with normal-density cells. This is compatible with a possibly higher expression of complement C3b receptors on the low-density eosinophils. Total arylsulfatase contents demonstrated that low-density eosinophils are not degranulated with respect to their small granules. Although release of the large granules by low-density eosinophils cannot be excluded, electron-microscopy studies indicated that degranulation is not the only (or major) factor that determines the density of the various eosinophilic subpopulations.
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Authors | H F Kauffman, B van der Belt, J G de Monchy, H Boelens, G H Koëter, K de Vries |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 79
Issue 4
Pg. 611-9
(Apr 1987)
ISSN: 0091-6749 [Print] United States |
PMID | 3558998
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Cell Count
- Eosinophilia
(blood)
- Eosinophils
(cytology, metabolism)
- Humans
- Hypersensitivity, Immediate
(blood)
- SRS-A
(biosynthesis)
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