Abstract |
Hepatocellular carcinoma (HCC), the fifth most common cancer worldwide, poses a severe threat to public health. Intraoperative fluorescence imaging provides a golden opportunity for surgeons to visualize tumor-involved margins, thereby implementing precise HCC resection with minimal damage to normal tissues. Here, a novel-acting contrast agent, which facilely bridges indocyanine green (ICG) and lipiodol using self-emulsifying nanotechnology, was developed for optical surgical navigation. Compared to clinically available ICG probe, our prepared nanoemulsion showed obviously red-shifted optical absorption and enhanced fluorescence intensity. Further benefiting from the shielding effect of lipiodol, the fluorescence stability and anti-photobleaching ability of nanoemulsion were highly improved, indicating a great capacity for long-lasting in vivo intraoperative imaging. Under the fluorescence guidance of nanoemulsion, the tumor tissues were clearly delineated with a signal-to-noise ratio above 5-fold, and then underwent a complete surgical resection from orthotopic HCC-bearing mice. Such superior fluorescence performances, ultrahigh tumor-to-liver contrast, as well as great bio-safety, warrants the great translational potential of nanoemulsion in precise HCC imaging and intraoperative navigation.
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Authors | Jing Zhu, Chengchao Chu, Dongsheng Li, Yang Zhang, Yi Cheng, Huirong Lin, Xiaoyong Wang, Junxian Liu, Xin Pang, Jingliang Cheng, Gang Liu |
Journal | Frontiers in bioengineering and biotechnology
(Front Bioeng Biotechnol)
Vol. 10
Pg. 890668
( 2022)
ISSN: 2296-4185 [Print] Switzerland |
PMID | 35547157
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Zhu, Chu, Li, Zhang, Cheng, Lin, Wang, Liu, Pang, Cheng and Liu. |