Anti-Hair Loss Effect of Adenosine Is Exerted by cAMP Mediated Wnt/β-Catenin Pathway Stimulation via Modulation of Gsk3β Activity in Cultured Human Dermal Papilla Cells.

Abstract

In the present study, we investigated the molecular mechanisms of adenosine for its hair growth promoting effect. Adenosine stimulated the Wnt/β-catenin pathway by modulating the activity of Gsk3β in cultured human dermal papilla cells. It also activated adenosine receptor signaling, increasing intracellular cAMP level, and subsequently stimulating the cAMP mediated cellular energy metabolism. The phosphorylation of CREB, mTOR, and GSK3β was increased. Furthermore, the expression of β-catenin target genes such as Axin2, Lef1, and growth factors (bFGF, FGF7, IGF-1) was also enhanced. The inhibitor study data conducted in Wnt reporter cells and in cultured human dermal papilla cells demonstrated that adenosine stimulates Wnt/β-catenin signaling through the activation of the adenosine receptor and Gsk3β plays a critical role in transmitting the signals from the adenosine receptor to β-catenin, possibly via the Gαs/cAMP/PKA/mTOR signaling cascade.

• Authors: Jaeyoon Kim, Jae Young Shin, Yun-Ho Choi, Nae Gyu Kang, Sanghwa Lee
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 27 Issue 7 (Mar 28 2022) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 35408582 (Publication Type: Journal Article)

Chemical References

• beta Catenin
• Glycogen Synthase Kinase 3 beta
• TOR Serine-Threonine Kinases
• Adenosine

Topics

• Adenosine (metabolism, pharmacology)
• Alopecia (metabolism)
• Cells, Cultured
• Glycogen Synthase Kinase 3 beta (genetics, metabolism)
• Hair Follicle (metabolism)
• Humans
• TOR Serine-Threonine Kinases (genetics, metabolism)
• Wnt Signaling Pathway
• beta Catenin (genetics, metabolism)